Lighting is the most important factor. It is important not only for the accumulation of astaxanthin, but also for the growth of Haematococcus pluvialis. Of course, astaxanthin can also be stacked at night, but the accumulation speed in the dark environment is 7 times lower than that in the case of light. The strong light can obviously suppress the growth of Haematococcus pluvialis to promote the accumulation of astaxanthin. Red light is beneficial to the growth of Haematococcus pluvialis, and blue light is more suitable for astaxanthin accumulation.
The effect of temperature on the accumulation of astaxanthin is similar to that of light. High temperatures will promote the accumulation of astaxanthin. According to experiments, the production value of Haematococcus pluvialis is 2.5 times that of 20 ° C at a temperature of 30 ° C. However, the best temperature for Haematococcus pluvialis to grow is 25 ° C-28 ° C. Haematococcus pluvialis is no longer produced above 30 ° C. Therefore, high temperature promotes the accumulation of astaxanthin, but it is only suitable for the later stage of Haematococcus pluvialis.
Experiments on the effect of stirring speed on Haematococcus pluvialis and astaxanthin showed that if the force was greater than 0.05N / M, the growth of Haematococcus pluvialis was inhibited, and astaxanthin accumulation was promoted.
4. Optimize the cultivation base
Acetate is a good source of nitrogen for cultivation, which can not only promote the growth of Haematococcus pluvialis, but also promote the accumulation of astaxanthin. However, with regard to the demand for nitrogen sources, most scientists think that low concentration of astaxanthin is advantageous for accumulation of astaxanthin. Some people also say that Haematococcus pluvialis is a good source of astaxanthin for accumulation of acetate in the cultivation base containing acetate. In addition to astaxanthin accumulation are also high concentration of ferrous ions, low concentration of phosphate.
5.Optimize environmental conditions
In fact, no matter what the environmental conditions, temperature or light, these are important for the accumulation of Haematococcus pluvialis and astaxanthin. But conditions that are suitable for one are often not suitable for the other. Suitable for the growth of Haematococcus pluvialis, the astaxanthin accumulation slows down; suitable for astaxanthin accumulation, the growth of Haematococcus pluvialis is unfavorable. Therefore, to optimize the environmental conditions, step cultivation can be used. First, let Haematococcus pluvialis have the best growth environment. After the purpose is achieved, changing the environment is a rapid accumulation of astaxanthin.